Monitoring Software - Thermo Scientific appliedbiosystems SeqStudio Flex Series User Manual

Appendix A Troubleshooting
A
Inspect the data—Troubleshooting procedures
Check size standard quality (fragment analysis) in the Remote Monitoring
software
This procedure describes how to check the size standard quality in the Remote Monitoring software.
Note: To determine the size standard that was used for a sample, view the run history for the sample
on the instrument.
1. In the Plates tab, select an injection group. Alternatively, you can click a row in the Run History
tab, then select a plate.
The electropherogram screen is displayed with the RAW tab selected.
The sample list displays QC and SQ values for each well. For information on how these values are
determined, see "Sample QC and quality alerts"
A QC status indicates all QC criteria were met. An SQ of >0.75 is a passing result.
If any wells have a
≤0.74, review the data for the well as described below.
478
or QC status (indicating that a quality alert was triggered), or an SQ of
SeqStudio ™ Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
on page 172.