Thermo Scientific appliedbiosystems SeqStudio Flex Series User Manual page 294

Chapter 11 Manage library resources on the instrument
11
Analysis settings library
Setting
Clear range methods
294
• Use quality values—Sets a window with a specified number of allowed
low-quality bases by removing bases until there are <X number of
bases per Z number of bases with QV <Y.
• Use base positions—Specifies the first and last basecalls in the range
to consider, or trims the specified number of basecalls from the 3' end.
• Use identification of N calls—Sets a window with a specified number
of allowed ambiguous basecalls (Ns) by removing bases until there are
<X number of Ns per Y number of bases.
SeqStudio ™ Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
Description