Thermo Scientific appliedbiosystems SeqStudio Flex Series User Manual page 320

Chapter 12 Run calibrations and install checks
12
Run a spectral calibration
Spectral QV (quality value) and Condition number definitions and limits
Spectral QV
A Spectral QV (quality value) reflects the confidence that the individual dye emission signals can be
separated from the overall measured fluorescence signal. It is a measure of the consistency between
the final matrix and the data from which it was computed. A Spectral QV of 1.0 indicates high
consistency, providing an ideal matrix with no detected pull-up/pull-down peaks.
In rare cases, a high Spectral QV can be computed for a poor matrix. This can happen if the matrix
standard contains artifacts, leading to the creation of one or more extra peaks. The extra peaks cause
the true dye peak to be missed by the algorithm, and can lead to a higher Spectral QV than would be
computed with the correct peak.
Condition number
A Condition number indicates the amount of overlap between the dye peaks in the fluorescence
emission spectra of the dyes in the dye set.
If there is no overlap in a dye set, the Condition number is 1.0 (ideal conditions), the lowest possible
value. The condition number increases with increasing peak overlap.
320
SeqStudio ™ Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide