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Chapter 12 Run calibrations and install checks
12
Perform an install run
Prepare the standard plate
IMPORTANT! Do not use warped or damaged plates.
1. Load the standards in any injection position in the plate.
The example below shows injection position 1, but you can specify the starting well for an injection
position. For information on other injection positions, see "Plate and strip-tube layout"
8-capillary
96-well plate
A1 through H1
1
2
A
1
B
2
C
3
4
D
E
5
F
6
G
7
H
8
2. Briefly centrifuge the plate that contains the standards.
3. Verify that each standard is positioned correctly in the
bottom of its well.
IMPORTANT! If the contents of any well contain
bubbles or are not located at the bottom of the well,
briefly centrifuge the plate, remove the plate from the
centrifuge, and verify that each standard is positioned
correctly in the bottom of its well.
4. Store the plate on ice until you prepare the plate assembly and load the plate in the instrument.
5. Prepare the plate assembly as described in "Prepare the plate assembly"
332
24-capillary
96-well plate
A1 through H1, A2 through
H2, and A3 through H3
1
2
3
4
A
1
2
3
4
5
6
B
7
8
9
C
10
11
12
D
13
14
15
E
F
16
17
18
G
19
20
21
H
22
23
24
SeqStudio
™
Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
24-capillary
384-well plate
Note: 384-well plates are not supported on 8‑capillary
instruments.
Columns 1, 3, and 5 in rows A, C, E, G, I, K, M, O
1
4
7
10
13
16
19
22
on page
2
3
5
6
8
9
11
12
12
14
15
12
17
18
12
20
21
12
23
24
12
on page
77.
75.
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Troubleshooting
