Thermo Scientific appliedbiosystems SeqStudio Flex Series User Manual page 345

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Reference sequence of the sequencing install standard
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Basecalls from the run. Basecalls that do not match the known bases in the reference sequence are marked in red.
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QV20 CRL is referred to as CRL on the Capillaries tab.
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Alignment range. The starting base is determined by the software. In the example above, the alignment range starts
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at 27 because of the contiguous bases before 27 that do not match the reference sequence.
Accuracy is referred to as %RL basecall on the Capillaries tab.
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Table 14 Plot view results—sequencing install run
Result
QV20 CRL (range)
Alignment
(This result is referred to
as Alignment read length
on the Capillaries tab.)
Signal strength
Accuracy
(This result is referred to
as % RL basecall on the
Capillaries tab.)
Install QC
SeqStudio ™ Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
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The same value that is displayed on the Capillaries tab.
The Alignment read length that is displayed on the Capillaries tab.
The average signal Intensity in the raw data.
The % RL basecall that is displayed on the Capillaries tab.
The same result that is displayed on the Capillaries tab.
Chapter 12 Run calibrations and install checks
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Description
12
Perform an install run
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