Theory Of Operation; Preparing Samples; Preparing The Instrument - Thermo Scientific appliedbiosystems SeqStudio Flex Series User Manual

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2
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Figure 5 Instrument rear panel connections
Recessed USB port
1
network adapter; adapter not shown)
USB port (for optional USB barcode scanner
2
connection)
Direct connection port
3
without a network)

Theory of operation

Preparing samples

When DNA samples are prepared for sequencing and fragment analysis on the instrument, fluorescent
dyes are attached to the DNA.

Preparing the instrument

The following runs are required to prepare the instrument for routine operation. After initial preparation
these runs are not required unless a new capillary array is installed or a new dye set/polymer
combination will be used.
• Spatial calibration—Associates the signal from each capillary to a specific position on the CCD
camera. For more information, see "Run a spatial calibration"
• Spectral calibration—Generates a matrix for each capillary that compensates for dye overlap and
is used to convert the 20–color data into 4-, 5-, or 6-dye data. For more information, see "Run a
spectral calibration"
• Install run—Ensures that the instrument meets specifications for the application types being run.
For more information, see "Perform an install run"
SeqStudio ™ Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
(for use with the wireless
(to allow computer connection
on page 310.
Chapter 1 Product information
Wired network port
4
Circuit breaker (rear power switch; use the power switch on
5
the front panel for normal operation)
Power receptacle
6
on page
on page 328.

Theory of operation

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