Perform An Install Run - Thermo Scientific appliedbiosystems SeqStudio Flex Series User Manual

Perform an install run

IMPORTANT! Ensure that polymer has been installed for <3 days before you run a fragment install
check. If you use older polymer, the install run may fail because allele peaks may not align with the
expected system-specified bins.
1. Load the install run plate in the instrument. See "Load a plate into the instrument"
2. In the home screen, tap
3. Tap the screen to select the plate type you are using for the install run.
4. Tap the injection group for the install standard in the plate, then tap Chemistry type.
5. Tap the Chemistry Type for install run.
Note: If you use the BigDye
see "Using BigDye
6. Tap Next.
The home screen is displayed with capillary information sharing settings.
7. (Sequencing install runs only) Select capillary information sharing settings:
• Use as spectral calibration—Uses the sequencing data for spectral calibration.
• Allow borrowing—Determines whether the software can use spectral calibration data from
an adjacent capillary if the data from a capillary does not meet the quality thresholds. For
information, see "Spectral calibration sharing between capillaries"
Retry function from spectral calibration is not performed if you allow borrowing for the install
run.)
SeqStudio ™ Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
Actions4Maintenance4Install run4Install run.
™
Direct Cycle Sequencing Kit, select BDTv3.1. For more information,
™
Direct Cycle Sequencing Kit chemistry"
Chapter 12 Run calibrations and install checks
on page 312.
on page
12
Perform an install run
on page 136.
321. (The Auto
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