Qc Errors-Sequencing Or Fragment Analysis Install Run - Thermo Scientific appliedbiosystems SeqStudio Flex Series User Manual

Appendix A Troubleshooting
A
QC error and alert troubleshooting
QC errors—Sequencing or fragment analysis install run
QC error
Sequencing
Failed the CRL specification.
Fragment analysis
Failed allele Bin specification.
494
Description
Low quality data, did not
meet Contiguous Read Length
requirements for an Install run.
Peaks have shifted outside the
software-defined bins.
SeqStudio ™ Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
Action
• Ensure that instrument
consumables are not expired
and have not exceeded
the on-instrument limits.
See "Instrument consumables
handling, usage limits, and
expiration" on page 25.
• Ensure that the capillary array
has not exceeded the number of
injections limit.
• Ensure the install standard
was prepared according to the
product information sheet.
• Ensure that the install standard
was prepared in the appropriate
™
volume of fresh Hi‑Di
Formamide to ensure adequate
signal. See "Hi‑Di ™ Formamide
storage and usage" on page
• Ensure that polymer has been
installed on the instrument for
<3 days.
• Ensure that instrument
consumables are not expired
and have not exceeded
the on-instrument limits.
See "Instrument consumables
handling, usage limits, and
expiration" on page 25.
28.