Chapter 3 Prepare The Samples; Sample Preparation Guidelines - Thermo Scientific appliedbiosystems SeqStudio Flex Series User Manual

3
■

Sample preparation guidelines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73

■
Plate and strip-tube layout . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
■
Prepare and assemble sample plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
Sample preparation guidelines
Item
Plates and tubes
Fragment analysis sample
preparation
SeqStudio ™ Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
• Use MicroAmp ™
Optical Reaction Plates or MicroAmp
base and retainer set. Use a base and retainer with the same catalog number.
• Use the appropriate septa for plates and tubes.
• See "Plates bases retainers and septa"
• Prepare the fragment analysis reactions as recommended by the kit instructions.
• Use a 10–20 µL sample volume for 96-well plates or 8-strip tubes. Use a 5–20 µL
sample volume for 384-well plates.
• Samples prepared with Hi‑Di
instrument. Samples that are prepared in aqueous solution have lower stability
than samples that are prepared in Hi‑Di
Note: Data quality may be reduced if samples exceed 16–24 hours on the
instrument. The software displays an alert if a sample will exceed 24 hours on
the instrument by the end of the run.
• Ensure that Hi‑Di ™
Formamide is fresh.
– Hi‑Di ™
Formamide should not undergo more than 8 freeze-thaw cycles. See
™
"Hi‑Di Formamide storage and usage"
– Use the same day after thawing.
• For more information, see DNA Fragment Analysis by Capillary Electrophoresis
User Guide (Pub. No. 4474504).
Prepare the samples
Guidelines
on page 539 for more information.
™ Formamide are stable for 16–24 hours on the
™
Formamide.
on page
™
Reaction Tubes with a
28.
73