Thermo Scientific appliedbiosystems SeqStudio Flex Series User Manual page 528

Appendix B Run modules and dye sets
B
Run modules, read lengths, size ranges, and run times
Table 17 Sequencing analysis run modules (continued)
Run module
type
Standard BDxStdSeq50_POP6
sequencing
BDxStdSeq50_POP6xl
BigDye
™
XTerminator
BDxStdSeq50_POP7
BDxStdSeq50_POP7xl
Microbial MicroSeqID50_POP6
sequencing
MicroSeqID50_POP6xl
for
™
MicroSEQ
ID kits
[1]
Throughput (samples/day): The total number of samples run in 23 hours (0.5 hour is required for user interaction and 0.5 hour is required for
warm-up time).
[2]
The maximum number of contiguous bases in the analyzed sequence with an average QV ≥20, calculated over a sliding window 20 base pairs
wide from an AB Long Read Standard sequencing sample. This calculation starts with base number 1. The read length is counted from the
middle base of the first good window to the middle base of the last good window, where a "good" window is one in which the average QV is
≥20.
528
Run module name
SeqStudio
Configuration
Cap.
Polymer
length
type
(cm)
™
50 POP-6
™
50 POP-7
™
50 POP-6
™ Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
[1]
23 hours throughput
Run
time (8‑cap.) (24-cap.)
(min)
≤140 ≥72 ≥216
≤125 ≥88 ≥264
≤135 ≥80 ≥240
Perform
- ance
Contig.
Read
Length
[2]
(CRL)
≥600
≥850
≥600