Sample And Data Troubleshooting - Thermo Scientific appliedbiosystems SeqStudio Flex Series User Manual

Appendix A Troubleshooting
A

Sample and data troubleshooting

Observation
Unable to connect to SAE
server
message
The Open file from non-SAE
system Setting from SAE
server setting in the SAE
Administrator Console does
not change the software
behavior

Sample and data troubleshooting

Observation
"Calibration from sample" is
reported for spectral calibration
status
"Calibration from manual" is
reported for spectral calibration
status, but no pull-up peaks
are observed
512
Possible cause
The SAE server connection
settings are incorrect.
There is a problem with
the computer on which the
SAE Administrator Console is
installed or a problem with the
network.
The computer on which the
SAE Administrator Console is
installed has a dynamic IP
address that is disconnecting
the server when the computer
is restarted.
Firewall settings are not
correctly set.
The Open file from non-
SAE system Setting from
SAE server function is not
supported in this software.
Possible cause
Auto-spectral calibration is run
on all samples and generates
the status.
See "Auto-spectral calibration
during an injection" on
page 150.
Auto-spectral calibration was
not used. For more information,
see "Auto-spectral calibration
during an injection" on
page 150.
SeqStudio ™ Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
Recommended action
1. Check the SAE server IP address (see
"Determine the IP address for a computer
on a network" on page
2. In the instrument Sign In screen, sign in
with a local administrator profile.
3. Set the correct IP address (in the home
screen, tap Actions4Settings4 SAE
settings4Connection settings).
Troubleshoot computer or network problems.
Set a static IP address on the computer.
Contact Technical Support.
No action.
Recommended action
No action. Normal occurrence.
No action. Normal occurrence.
443).