Thermo Scientific appliedbiosystems SeqStudio Flex Series User Manual page 322

Chapter 12 Run calibrations and install checks
12
Run a spectral calibration
Spectral calibration with borrowing enabled
Table 7 Overview of spectral calibration options with Allow borrowing enabled
Option
Allow borrowing enabled
Auto Retry disabled
(default)
Allow borrowing enabled
Auto Retry enabled
Table 8 Details of spectral calibration injections with Allow borrowing enabled and Auto Try enabled
Injection
Injection 1
Injection 2 (listed as
re-injection in
Run queue)
Injection 3 (listed as
re-injection in
Run queue)
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• All capillaries must pass (must meet the Spectral QV and Condition
number limits set internally in the software) and meet the borrowing limits
listed below.
– 8-capillary instruments—One adjacent-capillary borrowing event is
allowed
– 24-capillary instruments—Up to two adjacent-capillary borrowing
events are allowed.
• One run is performed (see Injection 1 below).
• If the limits are not met, the calibration fails.
This option is not illustrated in the table below.
Same as above, except up to 3 runs are performed if injections 1 and 2 fail (see
Injections 1 through 3 below).
Process
• The software evaluates the Spectral QV and Condition number of all capillaries.
• If all capillaries pass, the calibration is complete, and injections 2 and 3 are not
performed.
• If any capillaries fail, the software borrows from an adjacent capillary.
• If, after borrowing, >1 (8-capillary instruments) or > 2 capillaries (24-capillary
instruments) fail, injection 2 is performed.
• The software evaluates the Spectral QV and Condition number results between
adjacent capillaries in injection 2 and for each capillary across injections 1 and 2.
It uses the result with the highest Spectral QV for each capillary.
• If all capillaries pass, the calibration is complete and injection 3 is not performed.
• If, after borrowing, >1 (8-capillary instruments) or > 2 capillaries (24-capillary
instruments) from injection 1 or 2 do not pass, injection 3 is performed.
• The software evaluates the Spectral QV and Condition number results between
adjacent capillaries in injection 3 and for each capillary across injections 1, 2,
and 3. It uses the information with the highest Spectral QV for each capillary.
• If all capillaries now pass, the calibration passes.
• If after borrowing, >1 (8-capillary instruments) or > 2 capillaries (24-capillary
instruments) from injection 1, 2, or 3 do not pass, the calibration fails.
SeqStudio ™ Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
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