Thermo Scientific appliedbiosystems SeqStudio Flex Series User Manual page 6

Contents
Run plates with the automated barcode workflow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 147
View alert and notification details . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 149
Auto-spectral calibration during an injection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 150
View real-time results in the home screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 153
Pause the instrument in the home screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 157
Use the Run queue to monitor a run on the instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . 160
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CHAPTER 7 View results on the instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 169
Options for viewing results on the instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 169
View results for a completed run (Run history) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 170
Export results from the instrument-Results and data files, logs, and reports . . . . . . . . . 171
Sample QC and quality alerts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 172
Use the electropherogram screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 173
Sequence analysis results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 175
Fragment analysis results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 179
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CHAPTER 8 Use the Remote Monitoring software to monitor runs,
view results, and use cloud analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 182
Monitor a run from the Remote Monitoring software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 182
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Create the plate files before the barcoded plates are loaded into the drawer . . . . . 147
Pre-run checks and plate alerts in an automated barcode run . . . . . . . . . . . . . . . . . . 149
Auto-spectral calibration overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 150
Auto-spectral calibration requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 151
Auto-spectral calibration results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 152
Parts of the Run Queue screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 160
View real-time results in the Run Queue screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 162
Change the injection list order . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 163
Cancel or add injections or specify re-injections in the Run Queue screen . . . . . . . 165
Pause the instrument in the Run Queue screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 166
Parts of the sequencing electropherogram screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . 175
Sequencing results and well details . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 176
Understanding Quality Values (QVs) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 178
Parts of the fragment analysis electropherogram screen . . . . . . . . . . . . . . . . . . . . . . . 179
Fragment analysis results and well details . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 180
Open the Remote Monitoring software from the Plate Manager software (cloud) . 182
Open the Remote Monitoring software from the InstrumentConnect software . . . . 183
Parts of the Remote Monitoring software home screen . . . . . . . . . . . . . . . . . . . . . . . . 184
Plate file requirements for viewing analyzed and cloud results . . . . . . . . . . . . . . . . . . 187
View instrument notifications in your Thermo Fisher
Set up email notifications from the instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 189
View real-time results in the Remote Monitoring software . . . . . . . . . . . . . . . . . . . . . . 190
Sequencing results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 192
Fragment analysis results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 192
SeqStudio ™ Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
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Connect account . . . . . . . . . . 188