Appendix B Run Modules And Dye Sets; Run Modules, Read Lengths, Size Ranges, And Run Times - Thermo Scientific appliedbiosystems SeqStudio Flex Series User Manual

B
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Run modules, read lengths, size ranges, and run times . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 526

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Dye sets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 530
Run modules, read lengths, size ranges, and run times
IMPORTANT! Use BDX run modules only if you prepare samples with the BigDye XTerminator
Purification Kit. Use non-BDX run modules for samples purified with other methods.
Table 17 Sequencing analysis run modules
Run module
type
Short read ShortReadSeq50_POP7
sequencing
ShortReadSeq50_POP7xl
Short read BDxShortReadSeq50_POP7
sequencing
BDxShortReadSeq50_POP7xl
BigDye
™
XTerminator
Rapid RapidSeq50_POP6
sequencing
RapidSeq50_POP6xl
RapidSeq50_POP7
RapidSeq50_POP7xl
Rapid RapidSeq36_POP4
sequencing
RapidSeq36_POP4xl
RapidSeq36_POP6
RapidSeq36_POP6xl
RapidSeq36_POP7
RapidSeq36_POP7xl
526
Run modules and dye sets
Run module name
SeqStudio
Configuration
Cap.
Polymer
length
type
(cm)
50 POP-7 ™
™
50 POP-7
™
50 POP-6
™
50 POP-7
™
36 POP-4
36 POP-6 ™
36 POP-7 ™
™ Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
[1]
23 hours throughput
Run
time (8‑cap.) (24-cap.)
(min)
≤30 ≥368 ≥1,104
≤30 ≥368 ≥1,104
≤65 ≥168 ≥504
≤40 ≥280 ≥840
≤45 ≥240 ≥720
≤70 ≥168 ≥504
≤30 ≥368 ≥1,104
™
Perform
- ance
Contig.
Read
Length
[2]
(CRL)
≥300
≥300
≥450
≥500
≥400
≥600
≥600