Thermo Scientific appliedbiosystems SeqStudio Flex Series User Manual page 342

Chapter 12 Run calibrations and install checks
12
Perform an install run
Table 12 Capillaries tab—sequencing install run (continued)
Result
Alignment read length
% RL basecall
(This result is referred to
as Accuracy in the plot
view.)
Install QC
(This result is referred to
as QC on the info/details
screen.)
Details screen—sequencing install run
342
Length and range of the sample sequence in which basecalling accuracy is
>99% relative to the known reference sequence of the sequencing install
standard. It does not consider QV. It does consider the entire sequence.
The Alignment read length value is derived from basecall accuracy (% RL
basecall), and not from quality values.
The percent of basecalling accuracy within the alignment read length and range.
• —All QC tests passed.
•
—At least 1 warning quality alert was triggered.
•
—At least 1 failing quality alert was triggered.
For more information, see "Sample QC and quality alerts"
SeqStudio ™ Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
Description
on page 172