Perform An Install Run; Overview Of Install Checks - Thermo Scientific Applied Biosystems SeqStudio Genetic Analyzer User Manual

Perform an install run

Overview of install
checks
SeqStudio Genetic Analyzer Instrument and Software User Guide
™
Install checks are performed by a Field Service Engineer at the time of installation.
An install check can be run at any time with the following reagents to ensure
instrument performance:
Install check type
Sequencing
Fragment DS‑33 GeneScan
Size Standard v2.0 (Cat. No. 4376911)
HID GlobalFiler
kits:
Note: A J6 manual
dye calibration is
required before the
install check.
You can include multiple injection groups, multiple applications, and/or multiple
chemistries on an install run plate. For example, you can prepare an install run plate
that contains replictate injections of the fragment install standard and both
sequencing standards.
Figure 22 Example install run plate with multiple applications and replicate injections
Appendix C Dye calibration and install standard checks
• 3500/3500xL Sequencing Standards, BigDye
(Cat. No. 4404312)
• 3500/3500xL Sequencing Standards, BigDye
(Cat. No. 4404314)
™ Installation Standards with GeneScan
™ Allelic Ladder from one of the following installation
• SeqStudio ™ HID and Sequencing Installation Kit (Cat. No.
A46180)
• SeqStudio ™ HID Installation Kit (Cat. No. A46182)
Perform an install run
Reagent
™ Terminator v3.1
™ Terminator v1.1
C
™ 600 LIZ ™
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