Appendix E Run Modules; Run Modules, Read Lengths, Size Ranges, And Run Times - Thermo Scientific Applied Biosystems SeqStudio Genetic Analyzer User Manual

E

Run modules, read lengths, size ranges, and run times

Table 15 Sequencing run modules for standard sequencing
Run module
ShortSeq
ShortSeq_BDX
MediumSeq
MediumSeq_BDX
LongSeq
LongSeq_BDX
[1]
CRL was determined using the Long Read Sequencing standard. A minimum of 90% of analyzed sequences with an average QV ≥QV threshold
were observed.
Table 16 Fragment analysis run modules
Run module
SNaPshot
FragAnalysis
[2]
LongFragAnalysis
[1]
Resolution Range: The range of bases over which the resolution (peak spacing interval divided by the peak width at half-max in a GS600 or
GS1200 LIZ size standard sample sized with a third order fit) is ≥1. The table shows the resolution range in ≥90% of samples.
[2]
Load a maximum of 48 samples per plate if you use a long run module.
218
Contiguous read
[1]
length (CRL)
≥350
≥500
≥800
IMPORTANT! Use BDX run modules only if you prepare samples with BigDye
™
XTerminator Purification Kit. Use non-BDX run modules for samples purified with
other methods.
Resolution Approximate
range run time
40–120 bp 25 minutes
[1]
60–460 bp 45 minutes
[1]
60–600 bp < 2 hours
QV threshold
QV30
QV30
QV20
Sizing precision
40–120: <0.5
60–460: <0.15
60–460: <0.15
461–600: <0.3
601–800: >0.45
SeqStudio Genetic Analyzer Instrument and Software User Guide
™
Run modules
Approximate run
time
30 minutes
45 minutes
~ 2 hours
Compatible size
standards
GeneScan ™ 120 LIZ
Standard
All except GeneScan
1200 LIZ ™ Size Standard
™
• GeneScan
Size Standard v2.0
• GeneScan ™
LIZ ™ Size Standard
™ Size
™
™
600 LIZ
1200