Settings For Nucleic Acid Measurements; Calculations For Nucleic Acid Measurements - Thermo Scientific NanoDrop One User Manual

4 Nucleic Acid Applications
Measure dsDNA, ssDNA or RNA

Settings for Nucleic Acid Measurements

Setting
Baseline Correction

Calculations for Nucleic Acid Measurements

The Nucleic Acid applications use a
modification of the Beer-Lambert equation
(shown at right) to calculate sample
concentration where the extinction
coefficient and pathlength are combined
and referred to as a "factor."
30 NanoDrop One User Guide
To show the dsDNA, ssDNA or RNA settings, from the dsDNA, ssDNA or RNA
measurement screen, tap
Available Options
On or off
Enter baseline
correction wavelength
in nm or use default
value (340 nm)
> Nucleic Acid Setup.
Description
Optional user-defined baseline correction. Can
be used to correct for any offset caused by light scattering
particulates by subtracting measured absorbance at
specified baseline correction wavelength from absorbance
values at all wavelengths in sample spectrum. As a result,
absorbance of sample spectrum is zero at specified
baseline correction wavelength.
Extinction Coefficients vs Factors
Using the terms in the Beer-Lambert equation, factor (f) is
defined as:

factor (f) = 1/(
where:

= wavelength-dependent molar extinction coefficient in
ng-cm/µL
b = sample pathlength in cm
As a result, analyte concentration (c) is calculated as:

c = A * [1/( * b)]
or
c = A * f
where:
c = analyte concentration in ng/µL
A = absorbance in absorbance units (A)
f = factor in ng-cm/µL (see below)
* b)
Thermo Scientific