Hid Run Module, Dye Sets, Size Standard Definitions, And Kits - Thermo Scientific Applied Biosystems SeqStudio Genetic Analyzer User Manual

D
Appendix D Create and run an HID plate

HID run module, dye sets, size standard definitions, and kits

Item
Volumes of install check components
per reaction
Pass/fail criteria for HID J6 install
check

HID run module, dye sets, size standard definitions, and kits

Run module
HIDAnalysis
[1]
Resolution Range: The range of bases over which the resolution (peak spacing interval divided by the peak width at half-max in a GS600 size
standard sample sized with a third order fit) is ≥1.
[2]
The GeneScan ™ 500 LIZ ™ Size Standard was not included in the HID validation of the instrument.
Category
Dye sets
Size standards
Kits
216
• Size standard—0.4 µL
™
• Hi‑Di Formamide—9.6 µL
• Allelic ladder—1 µL
• 343 ladder peaks
• 26 size standard peaks
• Minimum peak height >400 RFU
• Standard deviation of the observed allele fragment sizes for all
4 capillaries: <0.15 base pairs (bp)
Resolution Approximate
range run time
[1]
60–470 bp 39 minutes
• G5 (DS-33)
• J6 (DS-36)
• J6-T (DS-37)
• GS500(-250)LIZ
• GS600_LIZ_(60-460)
• GS600_LIZ_(80-400)
• Setting of None disables the Marker-
to-Marker pull-up feature ("Kit" is
displayed when None is selected.
• GlobalFiler ™
• GlobalFiler ™ Express
• Huaxia ™ Platinum
™
• Identifiler Plus
• MiniFiler ™
Description
Sizing precision
60–470: <0.15
Items
• NGM Detect
• NGM SElect
• VeriFiler
• VeriFiler
• Yfiler
• Yfiler
SeqStudio Genetic Analyzer Instrument and Software User Guide
™
Compatible size standards
• GeneScan ™ 500 LIZ ™ Size
[2]
Standard
• GeneScan ™ 600 LIZ ™ Size
Standard v2.0
™
™
™ Express
™ Plus
™
™ Plus