Sequence Analysis Results - Thermo Scientific Applied Biosystems SeqStudio Genetic Analyzer User Manual

Sequence analysis
results
Sequencing results
Result
Quality
SeqStudio Genetic Analyzer Instrument and Software User Guide
™
Sequence analysis trace
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Quality Value bars and values:
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• Pure base with QV ³20
• Pure base with QV 15–19
• Pure base with QV <15
• Mixed base
Bases—Mixed bases are highlighted in red. You can set the secondary peak threshold, as a
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percentage of the primary peak, for consideration as a mixed base by the basecalling
algorithm. Reaching this threshold is a necessary but not sufficient condition for arriving
at a mixed base determination. See "Sequencing settings (base calling)" on page 129.
Trace color hide/show—Touch to open, then touch a color to hide or show.
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Analyzed trace
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Thumbnail trace—Drag the center of the pane in the thumbnail trace to display another
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trace area in the top pane. Drag the right or left handle of the pane to zoom horizontally.
Zoom tools—Touch to open.
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Next trace tool—Touch to view the raw trace or EPT for the well.
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QC flags
• —All QC tests passed.
• —At least 1 warning quality alert was triggered.
• —At least 1 failing quality alert was triggered.
Chapter 8 View and analyze results
View results on the instrument
Description
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