Nucleic Acid Reported Results - Thermo Scientific NanoDrop One User Manual

Micro-uv/vis spectrophotometer
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Nucleic Acid Reported Results

dsDNA measurement screen
Application
Sampling
method
Sample name;
select to edit
Measurement screen
Thermo Scientific
For each measured sample, the dsDNA, ssDNA and RNA applications show the UV
absorbance spectrum and a summary of the results. Below is an example of the
measurement screen of the PC control software:
Sample name of
next measurement;
select to edit
Nucleic acid
concentration
of PC Control software
Note Micro-volume absorbance measurements and measurements taken with
nonstandard cuvettes are normalized to a 10.0 mm pathlength equivalent.
Measure sample
Run Blank
UV spectrum
Purity ratios
4
Nucleic Acid Applications
Measure dsDNA, ssDNA or RNA
End experiment
Menu of options;
click to open
Right click graph
area to view
options
Menu of table options;
click to choose which
columns to report
Click row to select
sample and update
spectrum.
NanoDrop One User Guide
display
.
27

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