Prepare Samples And Blanks; Preparing Samples - Thermo Scientific NanoDrop One User Manual

9 Learning Center

Prepare Samples and Blanks

Prepare Samples and Blanks

Preparing Samples

204 NanoDrop One User Guide
• Isolate and purify samples before measuring them with the instrument.
Commercial sample isolation kits are available for these purposes, or use an
in-house protocol. After purification, analyte of interest is typically dissolved in
aqueous buffer solution before it is measured.
Tip: Any molecule that absorbs light at analysis wavelength will contribute to
total absorbance value used to calculate sample concentration.
• Ensure final analyte concentration is within instrument's
limits.
• For micro-volume measurements, gently (but thoroughly) vortex each sample
before taking a measurement.
Avoid introducing bubbles when mixing and pipetting. For more information, watch
multimedia training Effects of Bubbles in
Note Samples dissolved in extremely volatile solvent such as hexane may work
best with cuvette sampling option
5. Measure a sample:
– Fill clean cuvette to same height with
sample solution
– Replace blanking cuvette with sample
cuvette, making sure to align light paths
– Tap Measure
– Wait for measurement to complete
– Remove cuvette
– Clean cuvette according to
manufacturer specifications
Samples.
C
(NanoDrop One instruments only).
absorbance detection
Thermo Scientific