Thermo Scientific ISQ 7000 Hardware Manual page 62

1
Performing Routine Maintenance
Replacing a Column
34 ISQ 7000 Hardware Manual
i. Remove the notched septum from the column.
3. Set up the GC parameters:
a. Set the oven and injector temperature to 50 °C (122 °F).
b. Set the carrier gas flow to 1.0 mL/min.
c. Turn off vacuum compensation, which is located on the Carrier menu of the GC.
d. Use the column flowmeter connector to verify that there is flow through the column.
If you do not have a flowmeter, dip the column outlet in a small vial of methanol.
Bubbles indicate there is flow through the column. If there is no flow, check that the
carrier gas is on, the GC inlet is pressurized, and the column is not plugged. If there is
still no flow, consult the GC documentation or contact Technical Support.
e. Allow the column to purge for at least 10 minutes. If you used methanol to detect
column flow, remove column from methanol during purge time.
f. Insert the column into the fitting of the column flowmeter connector that blocks the
column flow.
4. Perform a column leak check:
a. On the TRACE 1310, select the Leak Check icon in the Maintenance menu.
Otherwise, perform the leak check through the Chromatography Data System. Refer
to the TRACE 1300 and TRACE 1310 Series GC User Guide for instructions.
b. Start the leak check.
The split and purge valves of the selected channel are automatically closed, and the
channel is pressurized with carrier gas to the leak check setpoint.
The system monitors the pressure for one minute. If the pressure does not drop more
than the maximum allowed sensitivity value, then the leak check will pass. If the leak
check does not pass, use the leak detector to find and fix any leaks.
Tip Leaks can be caused by not tightening the fitting on the column flowmeter
connector. Check the fitting before looking for the leak elsewhere.
CAUTION INSTRUMENT DAMAGE: Do not allow the column flowmeter
connector to exceed 80 °C (176 °F). Otherwise, it will melt and damage the
instrument.
ATTENTION DOMMAGES À L'INSTRUMENT : ne laissez pas le connecteur du
débitmètre de la colonne dépasser les 80 °C (176 °F). Dans le cas contraire, il va
fondre et endommager l'instrument.
c. Repeat the leak check until no leaks are indicated.
5. Calibrate the carrier gas flow (column evaluation):
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