Thermo Scientific ISQ 7000 Hardware Manual page 60

1
Performing Routine Maintenance
Replacing a Column
32 ISQ 7000 Hardware Manual
26. Insert the column into the MS transfer line and tighten the nut until the column just
resists sliding through the ferrule.
Figure 26. Installing the Column into the MS Transfer Line
27. Loosen the nut ¼ turn and gently push the column into the MS transfer line until it just
touches the V-lock source plug.
28. Pull the column ½ to 1 mm away from the V-lock source plug and tighten the nut ½
turn.
Note To avoid forcing the column into the V-lock source plug when tightening the nut,
the column should be pulled back approximately 1 mm before tightening the nut.
29. Ensure the column and nut are correctly installed in the MS transfer line.
30. Remove the V-lock source plug with the insertion/removal tool.
31. Lower the handle to close the vacuum interlock.
32. Remove the source exchange tool.
33. Allow the V-lock source plug to cool.
34. Carefully remove the V-lock source plug from the insertion/removal tool using the source
plug holder.
Tip To avoid collecting dust on the V-lock source plug, store it in a closed container when
not in use.
35. Using the source holder, place the ion source on the insertion/removal tool and install the
ion source using the vacuum interlock.
Once the ISQ 7000 instrument is pumped down and able to scan, view air water spectra and
look for evidence of leaks with a large m/z 28 signal. If you observe a leak, stop scanning and
gently tighten the nut in small increments until no leaks appear when scanning.
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