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Data Processing Parameters
Kinetic calculations
Time to maximum rate / 2
188
Thermo Scientific SkanIt
•
Reaction - Define whether the reaction is supposed to produce
increasing or decreasing signal levels. There are three options for this
setting (see Maximum rate" on page 185):
•
Undefined - Searches for the absolute maximum rate and returns
the corresponding time as a positive number.
•
Increasing - Searches for the maximum increasing rate and returns
the corresponding time as a positive number. If no increasing rate
is found, the result is NaN" (not a number).
•
Decreasing - Searches for the maximum decreasing rate and returns
the corresponding time as a positive number. If no decreasing rate
is found, the result is NaN" (not a number).
•
Ignore first (n) readings - Ignores a defined number of points
(readings), counted from the first reading.
•
Ignore last (n) readings - Ignores a defined number of points (readings),
counted from the last reading.
•
Window (2...100) - The number of consecutive readings to use for
evaluation.
The time to maximum rate / 2 is the time taken to reach half of the
maximum rate. The maximum rate is determined as shown in Time to
maximum rate" on page 187. This rate is halved and the data is scanned to
determine the first rate, which is equal or exceeds this rate. The result is the
time when this found rate occurs.
®
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Software for Varioskan
User Manual
Thermo Fisher Scientific
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