Thermo Scientific Varioskan User Manual page 110

Step Parameters
TRF scanning
108 Thermo Scientific SkanIt
The TRF scanning step includes the following parameters:
• Fixed wavelength - Select either excitation or emission wavelength as
the fixed wavelength and enter its wavelength. The wavelength range is
200 to 840 nm for the excitation and 270 to 840 nm for the emission
wavelength.
• Scanning wavelengths - Select the starting and ending wavelengths
and the step size (scanning resolution) for the scanning measurement.
The wavelength range is 200 to 840 nm for excitation and 270 to
840 nm for emission.
• Excitation bandwidth [nm] - Select the bandwidth of the excitation
wavelength to be used in the measurement. Bandwidth is selected using
an excitation slit that has 5 nm and 12 nm options. The default value
is 12 nm. The emission bandwidth is permanently set to 12 nm and
cannot be changed.
Note To prevent any stray light from the excitation light from
interfering with the emission light, the difference between the
excitation and emission wavelengths should be at least the sum of
the bandwidths used. If the 5 nm excitation and 12 nm emission
(permanent) bandwidths are used, the difference between excitation
and emission wavelengths should be at least 17 nm. With the 12 nm
excitation and emission bandwidths the difference should be at
least 24 nm.
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