Thermo Scientific Varioskan User Manual page 90

Protocols
Well loops
Example of a well loop in a
kinetic dispense and
fluorometric measurement
88 Thermo Scientific SkanIt
• Settle delay [ms] - Define the time delay before the instrument performs
the following reading after the plate has moved to a new reading position.
The range is 0 to 10,000 ms; the default value is 0 ms. For more
information, see Editing a protocol" on page 81.
• Execution block - Define the general order in which the steps are
applied to the individual wells on the plate. See the example below.
Note Spectral scanning and kinetic loop with dispense and
measurement steps are always performed for one well at a time,
regardless of the selections in the Execution block.
• Type - Select the group for which each step is executed before
moving to the next group. The available options are Plate and Well.
The default selection is Plate.
When Plate is selected, the first step is performed for the whole
plate, then the second step for the whole plate and so on.
When Well is selected, all steps under the well loop are performed
for the number of wells defined. Use the Well count parameter to
define how many wells you want to measure at a time.
• Execution order - Select the direction in which the steps are
executed on the plate. It also sets the direction in which the points
inside a well are measured in a multipoint measurement. The black
dot designates the starting point and the arrows show the execution
order (Figure 6-1). Use the up and down arrows to switch between
the different options. Use an execution order that is aligned to the
defined measurement area(s).
The following 24-well plate will be measured with a kinetic dispense and
fluorometric measurement with dispense in reading 1:
A1 A2
B1 B2
C1 C2
D1 D2
The following settings are selected:
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Software for Varioskan User Manual
A3 A4 A5 A6
B3 B4 B5 B6
C3 C4 C5 C6
D3 D4 D5 D6
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