Thermo Scientific Varioskan User Manual page 100

Step Parameters
Using pathlength correction in a measurement
98 Thermo Scientific SkanIt
Select the appropriate value and click OK. If the solvent is not on the list,
you can add a known K-factor to the database (see Editing pathlength
correction K-factors" on page 247) or measure the pathlength K-factor (see
Measuring new pathlength correction K-factors" on page 73).
An additional photometric measurement step (PLC) is automatically added
after the measurement step for determining the pathlength correction of
the selected solvent. You cannot delete this measurement step unless you
remove the pathlength correction. In a kinetic measurement with dispensing,
two PLC steps are added, one before dispensing and another after dispensing.
Note Readings numbered with 0 or negative numbers are
calculated using the PLC performed before the dispensing. Readings
numbered with positive numbers are calculated using the PLC
performed after the dispensing.
The chosen pathlength correction K-factor will be shown for the
measurement.
Add the pathlength correction for each measurement step separately.
Note If you use a pathlength correction factor which did not pass
the quality control check, the measurement results will be unreliable
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