Thermo Scientific Varioskan User Manual page 113

Thermo Fisher Scientific
• Dynamic range - Select the dynamic range to be used in the
measurement: AutoRange, Low, Medium Low, Medium High, or High.
For more information, see Dynamic range selections" on page 112.
• Measurement time [ms] - The measurement time defines the light
collection time used in the measurement. The wells are measured with
a selected measurement time. For more information, see Measurement
times" on page 113.
• Time Setting - Edit the TRF Decay time settings by clicking Edit....
You are asked to enter the values one by one. When you have given all
four values, the software may change the values, if they are not
compatible. You can use the Back and Next buttons to modify the
values. Click Finish to confirm the values.
• Integration time [µs] - Define the length of the measurement
window, that is, the time the emitted signal is collected for each
measurement cycle (see Figure 7-2).
• Start time [µs] - Define the time from the flash to the middle point
of the first measurement (see Figure 7-2).
• Stop time [µs] - Define the time from the flash to the middle point
of the last mesurement (see Figure 7-2).
• Step [µs] - Define the time difference between two sequential middle
points of the measurement (see Figure 7-2).
Thermo Scientific SkanIt
® ®
Software for Varioskan User Manual
Step Parameters
TRF decay
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