Measurement Times; Figure 7-3 Dynamic Range Selections In The Fi And Trf Measurements - Thermo Scientific Varioskan User Manual

Step Parameters

Measurement times

When selecting a fixed gain, the principle for achieving the best sensitivity
is to select the lowest possible range, without receiving overrange results in
the measurement.
The measured values are comparable regardless of the dynamic range
selection.
Figure 7-3. Dynamic range selections in the FI and TRF measurements
Measurement times
There is one xenon lamp flash for each 10 ms period of measurement time.
The amount of flashes can be set to 1 to 100 flashes per measurement (10
– 1,000 ms) for fluorescence intensity, photometric and TRF decay
measurements and 1 to 1,000 flashes per measurement (10 – 10,000 ms)
for TRF measurements. The amount of xenon lamp flashes affects the quality
of the measurement result. Thus, the more flashes, the better the quality of
the result.
Use the default measurement times which normally produce good results
(see Table 7-1). If there is a necessity to improve the quality of the results,
the flash amount should be increased. In photometric measurements, longer
than 100 ms measurement times are recommended to reduce noise if the
measured absorbance is too high.
The result is the mean value of individual 10 ms readings during the total
measurement time.
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Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual 113