Thermo Scientific Varioskan User Manual page 112

Step Parameters
TRF decay
110 Thermo Scientific SkanIt
The TRF decay step includes the following parameters:
• Excitation wavelength [nm] - Select the excitation wavelength to be
used in the measurement. The wavelength range is 200 to 840 nm; the
default value is 340 nm.
• Emission wavelength [nm] - Select the emission wavelength to be used
in the measurement. The wavelength range is 270 to 840 nm; the default
value is 615 nm.
• Excitation bandwidth [nm] - Select the bandwidth of the excitation
wavelength to be used in the measurement. Bandwidth is selected using
an excitation slit that has 5 nm and 12 nm options. The default value
is 12 nm. The emission bandwidth is permanently set to 12 nm and
cannot be changed.
Note To prevent any stray light from the excitation light from
interfering with the emission light, the difference between the
excitation and emission wavelengths should be at least the sum of
the bandwidths used. If the 5 nm excitation and 12 nm emission
(permanent) bandwidths are used, the difference between excitation
and emission wavelengths should be at least 17 nm. With the 12 nm
excitation and emission bandwidths the difference should be at
least 24 nm.
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