Thermo Scientific Varioskan Manuals

Manuals and User Guides for Thermo Scientific Varioskan. We have 1 Thermo Scientific Varioskan manual available for free PDF download: User Manual

Thermo Scientific Varioskan User Manual (324 pages)

multimode microplate reader Software

Brand: Thermo Scientific | Category: Laboratory Equipment | Size: 10.54 MB

Table of Contents
5
About the User Manual
17
Intended Users
17
For more Information
17
Warning and Other Markings Used in the Documentation
18
Chapter 1 Introduction
19
Skanit Software
19
Varioskan System
20
Figure 1-1 Operation Principle
20
Figure 1-2 Varioskan Spectral Scanning Multimode Reader
20
Chapter 2 Installing Skanit Software
21
Before Installation
21
Registering Skanit Software
21
Checking the PC Requirements
21
Table 2-1 Minimum PC Requirements
21
Microsoft Windows Settings
22
Letters Permitted with Skanit Software
23
Installing
23
Installing Software Prerequisites
25
Installing the Software
28
Installing DDE and RE Versions to One Computer
34
Installing Skanit Software for Different Instrument Models
34
Uninstalling Skanit Software
35
Reinstalling the Software
35
Communicating with Instruments
36
Adding an Instrument to the PC Database
36
Connecting the Instrument to the PC through a COM Port
39
Connecting the Instrument to the PC through a USB Port
40
Figure 2-1 Computer and Mains Power Supply Connectors in the Varioskan Instrument
40
Chapter 3 User Interface
43
Navigating in the Software
43
Protocol Editing Window
44
Figure 3-1 Different Parts of the Main Window
44
Plate Layout Editing Window
45
Figure 3-2 Main Window for Protocol Editing
45
Figure 3-3 Main Window for Plate Layout Editing
46
Results Viewing Window
47
Figure 3-4 Main Window for Results Viewing - Status Reports
47
Figure 3-5 Main Window for Results Viewing - Measurement Results as a
48
Figure 3-6 Main Window for Results Editing - Calculation Parameters
49
Figure 3-7 Main Window for Results Viewing - Calculation Results as a
49
Run Log Viewing Window
50
Figure 3-8 Main Window for Run Log Viewing
50
Exploiting the Software Features Fully
51
Lists
51
Figure 3-9 Arrow Showing the Sorting Order in Lists
51
Right-Click Menus
52
Entering Information Without Using the Mouse
52
Shortcuts
53
Tooltips
53
Table 3-1 Keyboard Shortcuts
53
Table 3-2 Function Key Shortcuts
53
Chapter 4 Sessions
55
General Operational Procedure
55
Figure 4-1 Session Structure
55
Launching Skanit Software
56
Managing Folders in the Tree View
57
Creating New Folders
57
Figure 4-2 Tree View in Skanit Software
57
Moving Folders or Sessions
59
Renaming Folders
59
Deleting Folders
60
Opening an Existing Session
61
Creating a New Session
63
Editing a Session
65
Saving a Session
65
Deleting a Session or a Run from the Database
66
Exporting and Importing Sessions
67
Exporting Sessions or Runs
67
Importing Sessions or Runs
68
Chapter 5 Executing a Session
71
Pathlength Correction
74
Measuring New Pathlength Correction K-Factors
75
Figure 5-1 Absorption Spectra of Water
75
Priming Dispensers
77
Emptying Dispensers
78
Washing Dispensers
79
Example of Dispenser Washing
80
Chapter 6 Protocols
83
Editing a Protocol
83
Adding New Protocol Steps
85
Figure 6-1 Available Execution Order Types
85
Deleting Protocol Steps
87
Area Definition
87
Well Loops
89
Measurement with Dispensing
91
Figure 6-2 Measurement after Dispense and Measurement Together with
92
Kinetic Loops
93
Figure 6-3 Interval Time, Total Time and Readings in a Kinetic Loop
93
Chapter 7 Step Parameters
95
Area Definition
95
Well Loop
95
Photometric Measurement
95
Photometric Spectrum Scanning
97
Using Pathlength Correction in a Measurement
99
Fluorometric Measurement
101
Defining Multiple Fluorometric Measurements
103
Fluorometric Spectrum Scanning
103
TRF Measurement
105
Defining Multiple TRF Measurements
108
Figure 7-1 TRF Principle
108
TRF Scanning
109
TRF Decay
111
Dynamic Range Selections
114
Figure 7-2 TRF Decay Definitions
114
Measurement Times
115
Figure 7-3 Dynamic Range Selections in the FI and TRF Measurements
115
Using Multipoint in a Measurement
116
Table 7-1 Measurement Times for each Measurement Technique and
116
Figure 7-4 Multipoint Example: Measurement Points in a Well and Their
117
Figure 7-5 Multipoint Selection: Center Point of a Well on a 48-Well Plate (A) and on a 6-Well Plate (B)
117
Kinetic Measurements
118
Incubate
118
Shake
119
Shaking Speed and Diameter Combinations
121
Figure 7-6 Shaking Sequence: on and off Time and Total Shaking
121
Time
121
Dispense
122
Figure 7-7 Recommended Shaking Speed and Diameter Combinations
122
Pause
124
Plate in
125
Plate out
125
Chapter 8 Plate Layouts
127
Working with Plate Layouts
127
Figure 8-1 Plate Layout Editing Window
128
Editing a Plate Layout
129
Adding Samples to the Plate Layout Using Fill Wizard
130
Adding a Concentration or Dilution Series
133
Editing Sample Properties and Adding Samples with Sample Properties
136
Adding New Samples to the Plate Layout
137
Creating New Groups for the Samples
139
Modifying Group Names
140
Changing Sample Types
140
Adding Additional Information to Replicates
141
Printing the Plate Layout
142
Chapter 9 Calculations
145
Adding Calculations
145
Deleting Calculations
147
Table 9-1 Required Data Reductions for Different Measurement Types
147
Chapter 10 Data Processing Parameters
149
Blank Subtraction
149
Examples of Blank Subtraction
150
Blank Subtraction in Kinetic Measurements
151
Basic Statistics
152
Data Normalization
153
Multipoint Calculation
154
Quantitative Curve Fit
155
Quantitative Curve Fit Definitions
155
Table 10-1 Number of Calibrators Required in Different Curve Fit Types
157
Polynomial Fit Types
158
Point to Point
159
Cubic Spline
159
Four Parameter Logistic and Log-Logit
160
Quantitative Curve Fit Results
160
Qualitative Classification
162
Figure 10-1 Dividing Measurement Results into Categories Using Cut-Off
162
Effective Dose
166
Figure 10-2 Effective Dose (ED) Determination
166
Quality Control (QC)
169
Creating Formulas for Quality Control (QC)
170
User-Defined Equation
171
Spectral Analysis
176
Spectral Peak Search
176
Figure 10-3 Spectral Peak Search: Threshold and Wavelength Area
177
Figure 10-4 Spectral Peak Search with Window Count 1
177
Spectral Maximum
178
Figure 10-5 Spectral Peak Search with Window Count 2
178
Spectral Minimum
179
HBW Calculation
180
Figure 10-6 Definition of the HBW Value
180
Figure 10-7 HBW Calculation in a Simple Case
181
Figure 10-8 HBW Calculation in a Spectrum with Two Peaks
181
Figure 10-9 Case Where an HBW Calculation Is Not Possible
182
Ratio Within Spectrum
183
Figure 10-10 Intensities I and I in Ratio Within Spectrum
183
Ratio between Spectra
184
Figure 10-11 Control and Sample Intensities in Ratio between Spectra
184
Select Wavelengths
185
Kinetic Calculations
185
Average Rate
186
Maximum Rate
187
Figure 10-12 Determining the Maximum Rate with Window Value 2
187
Time to Maximum Rate
189
Figure 10-13 Determining the Time to Maximum Rate
189
Time to Maximum Rate / 2
190
Time to Change
192
Maximum of Well (Peak)
193
Maximum - Minimum (Change)
194
Time to Maximum (Peak)
194
Time to Maximum (Peak) / 2
194
Select Reading
195
Ignore Readings
195
Integral
195
Sum
196
Figure 10-14 Integral Calculation
196
Average Value
197
Figure 10-15 Example of an Absorbance Curve
197
Baseline Subtraction
198
Figure 10-16 Determining the Average Value of a Kinetic Measurement
198
Figure 10-17 Determining the Baseline Subtracted Kinetic Curve
199
Merge Data
200
Graph
200
TRF Decay Calculation
203
Chapter 11 Results
205
Raw Measurement Data
205
Table 11-1 Result Information Shown in Result Lists
207
Calculation Results
208
Viewing Results in Table Format
208
Viewing Results in List Format
209
Viewing Results in Graph Format
210
Viewing Results as Curves
213
Saving Measurement and Calculation Data
213
Saving Automatically
213
Saving Manually
215
Measurement and Calculation Reports
215
Chapter 12 Instrument and Software Reports
221
Creating Instrument Reports
221
Viewing Software Reports
224
Viewing Run Status Reports
224
Chapter 13 Settings
227
Table 13-1 Available Settings Menu Items for Different Security Groups
227
Options
228
General
228
Database
229
Temperature
231
Number Formats
231
Email Settings
232
Table 13-2 Number Formats
232
Instrument
233
General
235
Communications
235
Dispensers
236
Reports
238
Colors
239
Plate Template Settings
240
Creating Plate Templates
241
Modifying Plate Templates
242
Plate Tray
244
Adding New Plate Trays to the Database
246
Associating Plate Templates with Plate Trays
247
Modifying Plate Trays
248
Editing Pathlength Correction K-Factors
249
Saved Curves
251
Protocol Template Setup
252
Skanit Control Panel
254
Users
255
Changing Your Own Logon Password
255
Viewing User Information
256
Groups
258
General Settings
258
Company Settings
259
Automation Interface
259
Chapter 14 Menus and Action Panels
261
Figure 14-1 Menus and Action Panels of Skanit Software
261
Menu Commands
262
Session
262
Edit
262
Steps
263
Data Processing
263
Settings
263
Electronic Signatures (DDE Only)
264
Execute
265
Help
265
Action Panels
266
Session Action Panel
266
Steps Action Panel
266
Results Action Panel
267
Report Toolbar
268
Execution Action Panel
268
Chapter 15 Using Help
269
Chapter 16 Troubleshooting Guide
271
Error Messages
271
Warning Messages
275
Appendix A Administrators
277
Skanit Control Panel
277
Users
277
Adding a New User
277
Modifying User Information
279
Unlocking User Accounts
280
Groups
281
Table A-1 Rights of the Different Security Groups
282
General Settings
284
Company Settings
284
Appendix B Database Maintenance
287
Backup
288
Restore
289
Archive
290
New Database
291
Upgrade
292
Changing the Working Database
292
Appendix C Drug Discovery Edition with 21CFR11 Compliance
295
Electronic Signature
295
Creating a Signature Password
296
Changing the Signature Password
299
Signing a Session or a Run
300
Viewing Signatures
301
Date and Time Format
302
Audit Trail
302
Glossary
305
Index
309

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