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Step Parameters
Dynamic range selections
Dynamic range
selections
112
Thermo Scientific SkanIt
Figure 7-2. TRF decay definitions
•
Optics - Fluorescence can be measured from one position:
•
Top - Fluorescence is measured at the measurement position above
the plate.
•
Bottom - This option is available with the Varioskan Flash only.
AutoRange (default) selects automatically that the optimal reading range is
used. It is based upon signal intensity in the well and uses the lowest possible
reading range to obtain best sensitivity.
However, if you need a faster reading speed or your kinetic reaction causes
overrange with the AutoRange setting, use the manual dynamic range
selection of Low, Medium Low, Medium High, or High range.
•
High range is intended for highest-concentration samples. It covers a
wide dynamic range with somewhat lower sensitivity than with other
dynamic ranges.
•
Low range produces on the other hand the highest sensitivity with a
limited dynamic range.
•
Medium Low range and Medium High range offer sensitivity and
dynamics in between the Low and High ranges.
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Software for Varioskan
User Manual
Thermo Fisher Scientific
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