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Data Processing Parameters
Multipoint calculation
Multipoint calculation
152
Thermo Scientific SkanIt
•
Source - Select which sample is used as the B
contains that sample. The mean value of the selected sample (if replicates
do exist) will be used in the calculation.
The B
and you can define which specific sample should be used as B
example, Cal_5 or Unknown_56. The ratio or inhibition value is
calculated for all the samples belonging to a certain group.
Multipoint calculation is used to reduce multiple measurement points in
each well to just one point per well (see Using multipoint in a
measurement" on page 114). Multipoint calculation must be performed
before any other data reduction, except Basic Statistics or Blank Subtraction,
is attempted. Multipoint calculation is only available for 6- to 48-well plates.
Select Data Processing > Multipoint Calculation, click the
the Results action panel, or right-click the measurement in the Results tree.
•
Average - The average value of all the measurement points in each well.
•
Minimum - The minimum value of all the measurement points in each
well.
•
Maximum - The maximum value of all the measurement points in each
well.
•
Maximum - Minimum - The minimum value of all the measurement
points in each well is subtracted from the maximum value of all
measurement points in the same well.
•
Standard deviation - The standard deviation of all the measurement
points in each well.
•
Sum - The sum of all the measurement points in each well.
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Software for Varioskan
User Manual
can be any sample type (calibrator, control, blank or unknown),
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and the group that
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, for
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button on
Thermo Fisher Scientific
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