Using Multipoint In A Measurement; Table 7-1 Measurement Times For Each Measurement Technique And - Thermo Scientific Varioskan User Manual

Step Parameters

Using multipoint in a measurement

Using multipoint in a
measurement
114 Thermo Scientific SkanIt
Table 7-1. Measurement times for each measurement technique and mode
Measurement
technique
Absorbance
(photometric)
Fluorometric
intensity (FI)
Time-resolved
fluorescence (TRF)
TRFdecay
In a multipoint well measurement, different parts of a well can be measured
separately. Multipoint measurement is only available for 6 to 48-well plates.
If the multipoint measurement is not selected, only the middle point of the
well is measured.
You can define which points are measured in the wells. The multipoint
pattern is only defined for the current step and is applied to all the wells
that are measured with that step. Points inside the well are measured in the
same direction as defined with the Execution order setting.
The measurement points in a well are equidistant from each other. The
location and distance of a single measurement point is measured from the
origin. The origin (0:0) is in the center of the well. The location is shown
as a coordinate value on the X and Y axes. A negative or positive value
indicates the direction from the origin. The distance is shown in units of
0.01 mm. The coordinates of measurement points are shown in the list
format of the measurement results.
In Figure 7-4, the measured points are orange. Their coordinates are shown
as values on the X and Y axes. For example, 190 : 0 means 1.90 mm left
in the X direction from the origin.
® ®
Software for Varioskan User Manual
Mode
Default
(Recommended)
Fixed 100
wavelength
Scanning 100
wavelength
Fixed 100
wavelength
Scanning 100
wavelength
Fixed 1000
wavelength
Scanning 1000
wavelength
100
Measurement time (ms)
min. max.
10 1,000
10 1,000
10 1,000
10 1,000
10 10,000
10 10,000
10 1,000
Thermo Fisher Scientific