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Chapter 4 Sessions; General Operational Procedure; Figure 4-1 Session Structure - Thermo Scientific Varioskan User Manual

Multimode microplate reader software

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General operational
procedure
Thermo Fisher Scientific
Chapter 4
Sessions
A session defines the protocol and plate layout that are required for a
successful execution of the session (Figure 4-1). The protocol consists of a
plate template, step parameters, and data processing parameters. Plate
template defines the plate type, for example, a 96-well plate. Step parameters
define all the control steps for the instrument, for example, measurement
techniques and dispensing. Data processing parameters define the
calculations applied to the measurement data. The plate layout consists of
the samples and their properties. Samples, such as blanks, controls and
unknowns, are defined to specific wells on the plate. Samples can further
be grouped and their properties, such as concentrations or dilutions, defined.
You can create a new session, save the current session and load an existing
one. Only one session can be loaded at a time.
A single execution of a session is called a run. A run produces measurement
data which is processed with the data processing parameters defined in the
session. A session can be executed several times, each of which results in a
new run and new measurement data. The runs of a session are saved under
the session and they can be opened or exported separately.
Figure 4-1. Session structure
The general operational procedure with SkanIt Software is as follows:
Launch the SkanIt Software application.
Load an existing session or create a new one.
Thermo Scientific SkanIt
®
®
Software for Varioskan
User Manual
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