Guidelines For Estimating Run Time - Thermo Scientific invitrogen E-Gel G8121ST User Manual

Guidelines for estimating run time

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E-Gel 1 Kb Plus Express DNA Ladder migration pattern
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Ladder
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Refer to the E-Gel 1 Kb Plus Express DNA Ladder migration pattern table to estimate
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target DNA run time to the reference line.
The run times indicated in the table are estimates. Monitor your gel in real time during
the run to ensure the sample does not pass the recovery well.
Identically sized bands in different wells may migrate differently.
DNA fragment size, amount, and salt content can affect migration rates.
Fragment size
5000 bp
3000 bp
2000 bp
1500 bp
1000 bp
750 bp
500 bp
300 bp
100 bp
DNA amount (per 25 μL)
100 ng
100 ng
100 ng
160 ng
90 ng
90 ng
180 ng
90 ng
90 ng
E-Gel Power Snap Electrophoresis System User Guide
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Migration time to
reference line
~27.5 min
~23 min
~20.5 min
~19 min
~17 min
~16 min
~15 min
~14 min
~13 min