Thermo Scientific Varioskan User Manual page 9

Chapter 8
Chapter 9
Chapter 10
Thermo Fisher Scientific
Plate In .............................................................................................. 123
Plate Out ........................................................................................... 123
Plate Layouts ..................................................................................... 125
Working with plate layouts ................................................................ 125
Editing a plate layout ......................................................................... 127
Adding samples to the plate layout using Fill Wizard ...................... 128
Adding a concentration or dilution series ........................................ 131
Editing sample properties and adding samples with Sample
Properties .......................................................................................... 134
Adding new samples to the plate layout ........................................ 135
Creating new groups for the samples ............................................. 137
Modifying group names ................................................................ 138
Changing sample types ................................................................. 138
Adding additional information to replicates .................................. 139
Printing the plate layout .................................................................... 140
Calculations ...................................................................................... 143
Adding calculations ........................................................................... 143
Deleting calculations ......................................................................... 145
Data Processing Parameters ............................................................. 147
Blank subtraction .............................................................................. 147
Examples of blank subtraction ......................................................... 148
Blank subtraction in kinetic measurements ...................................... 149
Basic statistics .................................................................................... 150
Data normalization ............................................................................ 151
Multipoint calculation ....................................................................... 152
Quantitative curve fit ......................................................................... 153
Quantitative curve fit definitions ..................................................... 153
Polynomial fit types ......................................................................... 156
Point to point .................................................................................. 157
Cubic spline .................................................................................... 157
Four parameter logistic and Log-Logit ............................................. 158
Quantitative Curve Fit results ......................................................... 158
Qualitative classification .................................................................... 160
Effective dose ..................................................................................... 164
Quality control (QC) ........................................................................ 167
Creating formulas for Quality Control (QC) .................................. 168
User-defined equation ....................................................................... 169
Spectral analysis ................................................................................. 174
Spectral peak search ......................................................................... 174
Spectral maximum .......................................................................... 176
Spectral minimum ........................................................................... 177
HBW calculation ............................................................................ 178
Ratio within spectrum ..................................................................... 181
Ratio between spectra ...................................................................... 182
Select wavelengths ........................................................................... 183
Kinetic calculations ............................................................................ 183
Average rate ..................................................................................... 184
Maximum rate ................................................................................ 185
Thermo Scientific SkanIt
® ®
Software for Varioskan User Manual
Contents
7