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User-defined equation
Thermo Fisher Scientific
•
Samples - Select a sample to be used in the formula. The list includes
all the available sample names and replicate names with replicate IDs
within the selected group.
•
Values - Enter the formula by using the number and operator buttons
and the Groups and Samples boxes. The
button for deleting one character at a time, and the
clearing the entire formula.
•
Formula - The formula is visible here in the way it will be shown in
the Formula field.
Click OK to accept the formula. The software attempts to verify that the
created formula is valid.
Note The software only checks the validity of the formula, not
the validity of the samples. Since it is possible to measure different
areas from the layout with different wavelengths, different number
of points in each well and different number of loops in kinetic
loops, the actual result from the calculation may be invalid.
The user-defined equation allows you to define basic calculations to calculate
data from different measurements, calculations or the same measurement
with a different wavelength. It is used, for example, to reduce data from
dual measurements or to calculate basic calculations, such as the purity of
DNA, using for instance 50 * (the absorbance at 260 nm).
In a kinetic measurement, the calculation is made for each reading.
Note The user-defined equation is always saved with the protocol.
It is not possible to make a user-defined equation in one protocol
and use the same user-defined equation in another protocol.
If you want to be able to use all the measurement steps and data processing
steps in the user-defined equation, place the User-Defined Equation step on
the root level (that is, select the session name from the Results tree and add
the User-Defined Equation step). If you want to use only the data from one
measurement or data processing step, select that step from the Results tree.
To add a user-defined equation step, select Data Processing > User-Defined
Equation, click the
the measurement in the Results tree.
Thermo Scientific SkanIt
button on the Results action panel, or right-click
®
Software for Varioskan
Data Processing Parameters
User-defined equation
button is the backspace
button is for
®
User Manual
169
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