Thermo Scientific Varioskan User Manual page 173

Functions in user-defined
Thermo Fisher Scientific
Note User-Defined Equation calculates the SD, CV and AVG
functions differently from Basic Statistics. The user-defined equation
calculates the average, SD or CV of all replicates and dilutions of
the sample. The basic statistics separates the dilutions and only
calculates the averages, SD or CV values of their respective
replicates. See the example at the end of this section.
The
a time, and the
• With dilutions - Select if dilutions for unknowns should be taken into
account in the data processing. If dilutions have already been used (for
example, in a previous curve fit calculation), they will not be calculated
again.
• Formula - The entered formula is visible here.
• Notes - You can add an optional description.
The software verifies that the created formula is valid.
Note The different variables are assumed to have an equal amount
of measurements. If there is not an equal amount of measurements
in different variables, the data processing result is invalid.
Note The software only checks the validity of the formula, not
the validity of the measurements. Since it is possible to measure
different areas from the layout with different measurement steps,
different number of points in each well and different number of
loops in kinetic loops, the actual result from the data processing
may be invalid.
This section describes the functions you can use in the formula of a
equation
user-defined equation. After selecting a function, define the data source
parameters: step, wavelength, group, sample, and column. The effect of
group and sample combinations are described for each function.
• ABS - Calculates the absolute values of negative values in the results.
To remove every negative value from the data, select [all groups in session]
from the Group menu and [all samples in group] from the Sample menu.
To remove negative values of a specific group, select the group from the
Group menu and [all samples in group] from the Sample menu.
• SQRT - Calculates the square root of the selected results. To calculate
square root from the data, select [all groups in session] from the Group
menu and [all samples in group] from the Sample menu. To calculate
button is the backspace button for deleting one character at
button is for clearing the entire formula.
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Thermo Scientific SkanIt Software for Varioskan
Data Processing Parameters
User-defined equation
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User Manual 171