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TRF measurement
Thermo Fisher Scientific
excitation and emission bandwidths the difference should be at
least 24 nm.
•
Dynamic range - Select the dynamic range to be used in the
measurement: AutoRange, Low, Medium Low, Medium High, or High.
For more information, see Dynamic range selections" on page 112.
•
Measurement time [ms] - The measurement time defines the light
collection time used in the measurement. The wells are measured with
a selected measurement time. For more information, see Measurement
times" on page 113.
•
Lag time [hh:mm:ss.t] - Define the time before the measurement is
executed.
•
Step duration [hh:mm:ss] - Define the total time for the step. The
software waits after the instrument has performed the action until the
time is up before proceeding with the following step.
•
Optics - Fluorescence can be measured from one position:
•
Top - Fluorescence is measured at the measurement position above
the plate.
•
Bottom - This option is available with the Varioskan Flash only.
The TRF Measurement step is used for measuring time-resolved fluorescence
using special TRF labels at selected excitation and emission wavelengths.
Select Steps > TRF or click the
The TRF measurement step includes the following parameters:
Thermo Scientific SkanIt
button on the action panel.
®
®
Software for Varioskan
User Manual
Step Parameters
TRF measurement
103
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