Ratio Between Spectra; Figure 10-11 Control And Sample Intensities In Ratio Between Spectra - Thermo Scientific Varioskan User Manual

Data Processing Parameters
Spectral analysis

Ratio between spectra

182 Thermo Scientific SkanIt
You can use spectral peak search (see Spectral peak search" on page 174)
to identify the wavelengths.
Note User-selectable wavelength values are not checked against
real measured values. Therefore, you may select wavelength values
that are not even measured.
Ratio between spectra calculates the ratio curve over a range of two
wavelengths. All the sample intensities of a certain wavelength n are divided
by the control intensity corresponding to the same wavelength n. The ratio
between spectra can be used to calculate the signal to blank spectra.
Figure 10-11. Control and sample intensities in ratio between spectra
The formulas for the calculation are as follows:
• Wavelength 1 - Select the starting wavelength to be used in the Ratio
between spectra calculation.
• Wavelength 2 - Select the end wavelength to be used in the Ratio
between spectra calculation.
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