Using Pathlength Correction In A Measurement - Thermo Scientific Varioskan User Manual

Using pathlength
correction in a
measurement
Thermo Fisher Scientific
• Pathlength correction - Tick the check box to launch the Pathlength
Correction K-Factors dialog. For more information, see Using
pathlength correction in a measurement" on page 97.
Pathlength correction calculates the measurement value from each well in
the microplate so that it corresponds to a measurement in a 10 mm cuvette.
Note that these factors are dependent on the solvent and the polymer used
for the plastic plate. If a new solvent or a new type of plate is used, then you
must generate a new K-factor.
Note If you have added a pathlength correction to a photometric
measurement and you want to remove the photometric
measurement, you must untick the Pathlength correction check box
first.
Note The pathlength correction is applicable only to water based
solutions. There must be an aqueous solution in the well when you
measure pathlength correction.
Note The pathlength correction is dependent on the material used
to manufacture the microplate, and should be determined each
time the plate type is changed (see Measuring new pathlength
correction K-factors" on page 73).
Note The pathlength correction factor can only be used with
either a 96-well or a 384-well plate.
Note A background subtraction step is automatically added to
the pathlength correction calculation. Therefore, blanks are needed
in the plate layout.
Tick the Pathlength correction check box. The Pathlength Correction
K-Factors dialog is launched.
Thermo Scientific SkanIt

Using pathlength correction in a measurement

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Software for Varioskan User Manual
Step Parameters
97