Thermo Scientific Varioskan User Manual page 76

Executing a Session
Pathlength correction
74 Thermo Scientific SkanIt
3. Select Protocol and follow the instructions given in the Protocol
description field in Protocol Properties. When you select Plate Layout,
the Description field at the bottom of the window provides information
on how to fill the wells of the plate with the desired solvent.
4. Make sure the instrument is connected to the software and save the
session. Click the
click the
and click OK. The Execution Progress window is launched and the
measurement starts.
5. After the measurement the result of the K-factor calculation is shown.
Validity checks:
The validity of the measurement data is checked automatically
according to the following quality control rules. Select K-Factor in
the Results tree. If the check passed, you see OK on the main window.
If the check did not pass, you see NOT OK.
•
•
•
6. Click the Add to Solvent List button. The Add New Pathlength
Correction Factor dialog is shown.
® ®
Software for Varioskan User Manual
button. Insert the plate into the instrument and
button. Give a name to the run in the Run Name dialog
Small volume: ΔA > 0.030
v1
• where ΔA is the absorbance difference between 975 nm and
v1
900 nm with volume 1
Large volume: ΔA = 2 * ΔA
v2
• where ΔA is the absorbance difference between 975 nm and
v2
900 nm with volume 2
CV%: After the session is run, four sets of data are created: vol1
with 900 nm, vol2 with 900 nm, vol1 with 975 nm, and vol2 with
975 nm. The calculated coefficient of variation (CV%) of each set
of data must be less than 10%.
± 10%
v1
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