Advertisement
Working with plate
Thermo Fisher Scientific
Chapter 8
Plate Layouts
A plate layout is used for telling the software how to handle the measurement
results in the calculations according to the different sample types: unknowns,
blanks, calibrators or standards, controls, and empty wells.
•
Unknown samples are the samples under investigation.
•
Blanks are used for blank subtraction.
•
Calibrators are samples with known concentrations used for quantitative
curve fit (see Quantitative curve fit" on page 153).
•
Controls are used for quality control (see Quality control (QC)" on
page 167) or for qualitative classification (see Qualitative classification"
on page 160).
•
Empty wells are kept empty and unavailable for the filling process.
You must define a plate layout before you can execute a measurement. If
you do not need a layout (if you only need to obtain raw measurement
data), tick the Fill layout with Unknowns check box when creating a new
session. Alternatively, you can also right-click on the plate layout and select
Fill Plate With Unknowns from the menu (see General operational
procedure" on page 53).
Samples of the same type have the same functionality in the assay, but their
concentration/dilution, name and other parameters may differ. Samples can
consist of individual replicates, each occupying one well on a microplate.
A plate layout can consist of several plates.
When you have created a new session as described in General operational
procedure" on page 53, you can start creating a new plate layout. Click
layouts
Plate Layout on the Session view to display the plate layout (Figure 8-1).
The plate layout is based on the selection made in the Create New Session
wizard.
®
Thermo Scientific SkanIt
Software for Varioskan
®
User Manual
125
Hide quick links:
Advertisement
