Table 10-1 Number Of Calibrators Required In Different Curve Fit Types - Thermo Scientific Varioskan User Manual

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3. Click Save data. Enter a name and description for the curve in the
Save Curve dialog. For example, enter the wavelength as a
description so that it is available for later use.
Click Save. For more information, see Saved curves" on page 249.
• Extrapolation - Tick the check box if you want to enable extrapolation.
Enter the minimum and maximum concentrations for the extrapolation.
Extrapolation is not available for point-to-point or cubic spline fit types.
Extrapolation is absolutely reliable only for the linear regression fit type,
due to mathematical reasons.
• Fit type - Select the quantitative curve fit type from the list. The curve
fit types are explained in Polynomial fit types" on page 156 through
Four parameter logistic and Log-Logit" on page 158. The number of
calibrators required for each curve fit type is shown in Table 10-1.
Table 10-1. Number of calibrators required in different curve fit types
Curve fit type
Linear regression (LLS)
Quadratic polynomial
Cubic polynomial
Quartic polynomial
Point to point
Cubic spline
Four parameter logistic
Log-Logit
• Transformation - Select the type of data transformation trailing.
Transformation of statistical data means in brief a change of scale used
Thermo Scientific SkanIt
Data Processing Parameters
Minimum number of calibrators
1 (3 is recommended - see the Note in
Polynomial fit types" on page 156)
3
4
5
2
3
4
4
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Software for Varioskan User Manual
Quantitative curve fit
155