Measuring New Pathlength Correction K-Factors; Figure 5-1 Absorption Spectra Of Water - Thermo Scientific Varioskan User Manual

Measuring new pathlength
correction K-factors
Thermo Fisher Scientific
Figure 5-1. Absorption spectra of water
The pathlength correction value (K-Factor) is different for different solvents
and should be determined separately for each solvent used.
Note The pathlength correction is dependent on the material used
to manufacture the plate, and should be determined separately for
each plate type.
Note The pathlength correction factor can only be used with 96-
and 384-well plates.
When you use a plate type and/or a solvent for the first time to measure
absorbances (photometric measurements) and you are going to perform a
pathlength correction, first define the pathlength correction K-factor using
a specially defined protocol in the software.
Note The pathlength correction is applicable only to water-based
solutions. There must be an aqueous solution in the well when you
measure pathlength correction.
1.
Create a new session (Session > New... or the
K factor for pathlength correction protocol type and the Pathlength
Correction template and give a name to the session. Click Next.
2. Select the correct plate type from the Plate layout options list and click
Next. Select a folder where the session is saved. Click Finish. A ready
protocol opens in the main window.
Thermo Scientific SkanIt
button). Select the
® ®
Software for Varioskan User Manual
Executing a Session
Pathlength correction
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