Thermo Scientific Varioskan User Manual page 123

Thermo Fisher Scientific
The dispensing step includes the following parameters:
• Dispenser - Select which dispenser will be used. Only one dispenser is
available with the Varioskan instrument. Also see Dispensers" on
page 234.
• Volume [µl] - Enter the volume to be dispensed into all the wells in
the defined area. The range is 1 to 1,000 µl in 1 µl increments. The size
of the well can affect the available upper limit. The default volume is
50 µl.
During the execution of a session, the total dispensing volume of each
well is calculated by summing up all the dispensed volumes into a well.
The execution of a session is prevented if the total dispensed volume
exceeds 80% of the well volume in any well.
• Dispensing Speed - Select the speed at which the instrument dispenses
the reagent into the wells.
The optimal speed depends on the viscosity of the dispensed solution.
Too low a speed prevents adequate mixing of solutions in the well, or
leaves the dispensed liquid as a droplet on the dispenser tip. Too high
a speed can cause the solution to splash outside the well. However, too
high a speed can also speed up the mixing of the dispensed liquid and
the solution in the well.
• Low - The instrument dispenses at a speed that is 10% of the
maximum speed.
Thermo Scientific SkanIt
® ®
Software for Varioskan User Manual
Step Parameters
Dispense
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