Thermo Scientific Varioskan User Manual page 175

Comparison of the
calculation of average in
User-Defined Equation and
Basic Statistics
Thermo Fisher Scientific
selected group. If you select a specific sample from the Sample menu,
the sample value or the replicate value is returned.
• SD - Calculates the standard deviation of the samples in the selected
group(s). Select either [all groups in session] or a specific group from the
Group menu and [all samples in group] from the Sample menu. The
calculation returns the standard deviation. If you select a specific sample
from the Sample menu, the sample value or the replicate value is
returned.
The formula of variance is used in calculating the SD, refer to Basic
statistics" on page 150.
• CV - Calculates the coefficient of variation of the samples in the selected
group(s). Select either [all groups in session] or a specific group from the
Group menu and [all samples in group] from the Sample menu. The
calculation returns the coefficient of deviation as a percentage. If you
select a specific sample from the Sample menu, the sample value or the
replicate value is returned.
User-Defined Equation calculates the SD, CV and AVG functions differently
from Basic Statistics. The user-defined equation calculates the average, SD
or CV of all replicates and dilutions of the sample. The basic statistics
separates the dilutions and only calculates the averages, SD or CV values of
their respective replicates.
This example demonstrates the difference when calculating the average
(AVG). The sample has two dilutions with three replicates each.
Un_0001_01 1/3
1:1 1.432
Un_0001_01 2/3
1:1 1.234
Un_0001_01 3/3
1:1 1.322
The basic statistics separates the dilutions and only calculates the averages
of their respective replicates when the Combine dilutions option is not
selected. The dilutions are not taken into account.
Un_0001_01 1/3
1:1 1.329
Both the user-defined equation and the basic statistics with the Combine
dilutions option selected calculate the average of all replicates and dilutions
of the sample. The dilutions are taken into account. All the dilutions of a
sample are multiplied by the dilution factor and the average is calculated.
Thermo Scientific SkanIt
Data Processing Parameters
Un_0001_02 1/3
1:2 0.723
Un_0001_02 2/3
1:2 0.711
Un_0001_02 3/3
1:2 0.692
Un_0001_02 1/3
1:2 0.709
® ®
Software for Varioskan User Manual
User-defined equation
173