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Thermo Fisher Scientific
•
Temperature [°C] - The target instrument temperature. The available
temperature range is from 10°C to 45°C. Temperatures below ambient
+ 4°C cannot be reached. The default value is 37°C.
•
Leave instrument at this temperature - When selected, the incubator
is left on at the defined temperature after the step ends. The temperature
is kept for the duration of the entire session or until the next incubation
step in the session. If there is another incubation step, the incubator is
heated up to the temperature defined in the step or it cools down
passively to the defined temperature. After the session ends, the incubator
reverts to the defined plate startup temperature or is switched off if no
startup temperature is defined (see Temperature" on page 229).
When not selected, the incubator is reverted to the previous set
temperature. For example, if the protocol has one Incubate step only
and the option is not selected, the incubator reverts to the plate startup
temperature. If the protocol has two Incubate steps and the first step
has the option selected but the second one does not, the incubator reverts
to the temperature set in the first step.
•
Wait until instrument has reached the target temperature - When
selected, the incubation time is only counted from the time that the
instrument has reached the target temperature. If not, also the time it
takes for the instrument to reach the target temperature is included.
Note It is possible to set the instrument temperature to be kept
at a defined temperature at all times. Select Settings > Options >
Temperature (see Temperature" on page 229).
Note The samples in the microplate usually reach the target
temperature much later than the instrument. For example, for a
covered 96-well plate with 200 µl water/well, the liquid warm-up
time from 25°C to 37°C takes approximately 1 h. The warm-up
time also depends on the liquid that is used.
Shake
The Shake step is used for shaking the microplate (orbital shaking) in order
to mix the samples.
®
Thermo Scientific SkanIt
Software for Varioskan
Step Parameters
Shake
®
User Manual
117
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